| THP

Tamm-Horsfall protein (THP), also known as uromodulin (UMOD), is a glycoprotein containing a transparent ribbon-like structure, encoded by the UMOD gene. It consists of an N-terminal EGF domain, two calcium-binding EGF domains, a cysteine-rich domain (D8C), a fourth EGF domain, and a C-terminal Zona pellucida-like (ZP) domain.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (μg/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.22	1.56	0.92	0.63	0.42	0.25	0.18	0.08
Corrected OD	2.14	1.48	0.84	0.55	0.34	0.17	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse THP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Urine(n=8)	84-101	95

 

| Linearity

Samples were spiked with high concentrations of Mouse THP and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Urine(n=4)
1 : 2	Range (%)	84-97
	Average (%)	93
1 : 4	Range (%)	89-105
	Average (%)	94